TY - JOUR
T1 - Triterpenoid acids and lactones from the leaves of Fadogia tetraquetra var. tetraquetra (Rubiaceae)
AU - Mulholland, Dulcie A.
AU - Mohammed, Abdelhafeez M.A.
AU - Coombes, Philip H.
AU - Haque, Shafiul
AU - Pohjala, Leena L.
AU - Tammela, Päivi S.M.
AU - Crouch, Neil R.
PY - 2011/11
Y1 - 2011/11
N2 - Four triterpenoids isolated from the leaves of Fadogia tetraquetra var. tetraquetra, 3β-hydroxy-11α,12α-epoxyoleanan-28,13β-olide (1), 3β-hydroxyurs-11-en-28,13β-olide (2), oleanolic acid (3), and ursolic acid (4), were evaluated for their antiviral and antibacterial properties. Compound 4 showed potent activity against the Semliki Forest virus with an IC50 of 14.7 μM, but was also found to be significantly cytotoxic (68% reduction in cell viability after 24 hours exposure at 50 μM) towards baby hamster kidney (BHK21) host cells. A viability assay on the mammalian human hepatocellular carcinoma (Huh-7) cell line showed no significant effects on intracellular ATP content after 48 hours exposure to compounds 1-4 at this concentration. Compound 4 also inhibited Staphylococcus aureus (MIC 12.5 μM), but was inactive against Enterobacter aerogenes, Escherichia coli, and Pseudomonas aeruginosa. Compounds 1-3 were inactive against all tested bacterial strains at 50 μM concentration.
AB - Four triterpenoids isolated from the leaves of Fadogia tetraquetra var. tetraquetra, 3β-hydroxy-11α,12α-epoxyoleanan-28,13β-olide (1), 3β-hydroxyurs-11-en-28,13β-olide (2), oleanolic acid (3), and ursolic acid (4), were evaluated for their antiviral and antibacterial properties. Compound 4 showed potent activity against the Semliki Forest virus with an IC50 of 14.7 μM, but was also found to be significantly cytotoxic (68% reduction in cell viability after 24 hours exposure at 50 μM) towards baby hamster kidney (BHK21) host cells. A viability assay on the mammalian human hepatocellular carcinoma (Huh-7) cell line showed no significant effects on intracellular ATP content after 48 hours exposure to compounds 1-4 at this concentration. Compound 4 also inhibited Staphylococcus aureus (MIC 12.5 μM), but was inactive against Enterobacter aerogenes, Escherichia coli, and Pseudomonas aeruginosa. Compounds 1-3 were inactive against all tested bacterial strains at 50 μM concentration.
KW - 3β-hydroxy-11α,12α- epoxyoleanan-28,13β-olide
KW - 3β-hydroxyurs-11-en-28,13β-olide
KW - Antibacterial
KW - Antiviral
KW - Cytotoxic
KW - Fadogia tetraquetra
KW - Oleanolic acid
KW - Rubiaceae
KW - Semliki forest virus
KW - Ursolic acid
UR - https://www.scopus.com/pages/publications/82455191741
U2 - 10.1177/1934578x1100601103
DO - 10.1177/1934578x1100601103
M3 - Artículo
C2 - 22224262
AN - SCOPUS:82455191741
SN - 1934-578X
VL - 6
SP - 1573
EP - 1576
JO - Natural Product Communications
JF - Natural Product Communications
IS - 11
ER -