Apoptosis induction in human hepatoma cell line HepG2 cells by trans- Anethole via activation of mitochondria-mediated apoptotic pathways

Steve Harakeh; Rajaa Al-Raddadi; Turki Alamri; Soad Al-Jaouni; Mohammed Qari; Yousef Qari; Ajay Kumar; Hanaa M. Tashkandi; Mohammed Moulay; Alia M. Aldahlawi; Petr Slama; Shafiul Haque

doi:10.1016/j.biopha.2023.115236

Apoptosis induction in human hepatoma cell line HepG2 cells by trans- Anethole via activation of mitochondria-mediated apoptotic pathways

Steve Harakeh
, Rajaa Al-Raddadi
, Turki Alamri
, Soad Al-Jaouni
, Mohammed Qari
, Yousef Qari
, Ajay Kumar
, Hanaa M. Tashkandi
, Mohammed Moulay
, Alia M. Aldahlawi
, Petr Slama
, Shafiul Haque

King Fahd Medical Research Center
King Abdulaziz University
Chandigarh University
Faculty of Sciences, King Abdulaziz University
Mendel University in Brno
Jazan University
Lebanese American University
Ajman University

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18 Citas (Scopus)

Resumen

trans-Anethole a valuable compound derived from star anise widely used by ethnic tribals to manage numerous human diseases. In this study antiproliferative activities of trans-Anethole towards human liver cancer (HepG2), cervical cancer (HeLa) and breast cancer (MCF-7) cells were explored. trans-Anethole showed free radical scavenging potential as assessed by DNA nicking assay. trans-Anethole exhibited strong antiproliferative potential towards HepG2 cells compared to other cell lines. trans-Anethole strongly induced apoptosis in HepG2 cells by significantly upregulating the protein expressions of p53, Caspase-3 and Caspase-9 were assessed by western blotting analysis which highlighted apoptosis-inducing capacity of trans-Anethole against HepG2 cells. Rt-qPCR analysis revealed that trans- Anethole upregulated p53, caspase − 3 and − 9 in comparison to untreated HepG2 cancer cells. Moreover, trans-Anethole provoked the generation of ROS and disruption of MMP. Our research suggests that trans-Anethole may have a significant anticancer therapeutic potential for treating liver cancer.

Idioma original	Inglés
Número de artículo	115236
Publicación	Biomedicine and Pharmacotherapy
Volumen	165
DOI	https://doi.org/10.1016/j.biopha.2023.115236
Estado	Publicada - sep. 2023
Publicado de forma externa	Sí

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Harakeh, S., Al-Raddadi, R., Alamri, T., Al-Jaouni, S., Qari, M., Qari, Y., Kumar, A., Tashkandi, H. M., Moulay, M., Aldahlawi, A. M., Slama, P., & Haque, S. (2023). Apoptosis induction in human hepatoma cell line HepG2 cells by trans- Anethole via activation of mitochondria-mediated apoptotic pathways. Biomedicine and Pharmacotherapy, 165, Artículo 115236. https://doi.org/10.1016/j.biopha.2023.115236

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title = "Apoptosis induction in human hepatoma cell line HepG2 cells by trans- Anethole via activation of mitochondria-mediated apoptotic pathways",

abstract = "trans-Anethole a valuable compound derived from star anise widely used by ethnic tribals to manage numerous human diseases. In this study antiproliferative activities of trans-Anethole towards human liver cancer (HepG2), cervical cancer (HeLa) and breast cancer (MCF-7) cells were explored. trans-Anethole showed free radical scavenging potential as assessed by DNA nicking assay. trans-Anethole exhibited strong antiproliferative potential towards HepG2 cells compared to other cell lines. trans-Anethole strongly induced apoptosis in HepG2 cells by significantly upregulating the protein expressions of p53, Caspase-3 and Caspase-9 were assessed by western blotting analysis which highlighted apoptosis-inducing capacity of trans-Anethole against HepG2 cells. Rt-qPCR analysis revealed that trans- Anethole upregulated p53, caspase − 3 and − 9 in comparison to untreated HepG2 cancer cells. Moreover, trans-Anethole provoked the generation of ROS and disruption of MMP. Our research suggests that trans-Anethole may have a significant anticancer therapeutic potential for treating liver cancer.",

keywords = "Antiproliferative, Apoptosis, DNA nicking, HepG2, Mitochondrial membrane potential (MMP), Reactive oxygen species (ROS)",

author = "Steve Harakeh and Rajaa Al-Raddadi and Turki Alamri and Soad Al-Jaouni and Mohammed Qari and Yousef Qari and Ajay Kumar and Tashkandi, \{Hanaa M.\} and Mohammed Moulay and Aldahlawi, \{Alia M.\} and Petr Slama and Shafiul Haque",

note = "Publisher Copyright: {\textcopyright} 2023 The Authors",

year = "2023",

month = sep,

doi = "10.1016/j.biopha.2023.115236",

language = "Ingl{\'e}s",

volume = "165",

journal = "Biomedicine and Pharmacotherapy",

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Harakeh, S, Al-Raddadi, R, Alamri, T, Al-Jaouni, S, Qari, M, Qari, Y, Kumar, A, Tashkandi, HM, Moulay, M, Aldahlawi, AM, Slama, P & Haque, S 2023, 'Apoptosis induction in human hepatoma cell line HepG2 cells by trans- Anethole via activation of mitochondria-mediated apoptotic pathways', Biomedicine and Pharmacotherapy, vol. 165, 115236. https://doi.org/10.1016/j.biopha.2023.115236

TY - JOUR

T1 - Apoptosis induction in human hepatoma cell line HepG2 cells by trans- Anethole via activation of mitochondria-mediated apoptotic pathways

AU - Harakeh, Steve

AU - Al-Raddadi, Rajaa

AU - Alamri, Turki

AU - Al-Jaouni, Soad

AU - Qari, Mohammed

AU - Qari, Yousef

AU - Kumar, Ajay

AU - Tashkandi, Hanaa M.

AU - Moulay, Mohammed

AU - Aldahlawi, Alia M.

AU - Slama, Petr

AU - Haque, Shafiul

PY - 2023/9

Y1 - 2023/9

N2 - trans-Anethole a valuable compound derived from star anise widely used by ethnic tribals to manage numerous human diseases. In this study antiproliferative activities of trans-Anethole towards human liver cancer (HepG2), cervical cancer (HeLa) and breast cancer (MCF-7) cells were explored. trans-Anethole showed free radical scavenging potential as assessed by DNA nicking assay. trans-Anethole exhibited strong antiproliferative potential towards HepG2 cells compared to other cell lines. trans-Anethole strongly induced apoptosis in HepG2 cells by significantly upregulating the protein expressions of p53, Caspase-3 and Caspase-9 were assessed by western blotting analysis which highlighted apoptosis-inducing capacity of trans-Anethole against HepG2 cells. Rt-qPCR analysis revealed that trans- Anethole upregulated p53, caspase − 3 and − 9 in comparison to untreated HepG2 cancer cells. Moreover, trans-Anethole provoked the generation of ROS and disruption of MMP. Our research suggests that trans-Anethole may have a significant anticancer therapeutic potential for treating liver cancer.

AB - trans-Anethole a valuable compound derived from star anise widely used by ethnic tribals to manage numerous human diseases. In this study antiproliferative activities of trans-Anethole towards human liver cancer (HepG2), cervical cancer (HeLa) and breast cancer (MCF-7) cells were explored. trans-Anethole showed free radical scavenging potential as assessed by DNA nicking assay. trans-Anethole exhibited strong antiproliferative potential towards HepG2 cells compared to other cell lines. trans-Anethole strongly induced apoptosis in HepG2 cells by significantly upregulating the protein expressions of p53, Caspase-3 and Caspase-9 were assessed by western blotting analysis which highlighted apoptosis-inducing capacity of trans-Anethole against HepG2 cells. Rt-qPCR analysis revealed that trans- Anethole upregulated p53, caspase − 3 and − 9 in comparison to untreated HepG2 cancer cells. Moreover, trans-Anethole provoked the generation of ROS and disruption of MMP. Our research suggests that trans-Anethole may have a significant anticancer therapeutic potential for treating liver cancer.

KW - Antiproliferative

KW - Apoptosis

KW - DNA nicking

KW - HepG2

KW - Mitochondrial membrane potential (MMP)

KW - Reactive oxygen species (ROS)

UR - https://www.scopus.com/pages/publications/85166235565

U2 - 10.1016/j.biopha.2023.115236

DO - 10.1016/j.biopha.2023.115236

M3 - Artículo

C2 - 37531780

AN - SCOPUS:85166235565

SN - 0753-3322

VL - 165

JO - Biomedicine and Pharmacotherapy

JF - Biomedicine and Pharmacotherapy

M1 - 115236

ER -

Apoptosis induction in human hepatoma cell line HepG2 cells by trans- Anethole via activation of mitochondria-mediated apoptotic pathways

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