TY - JOUR
T1 - Antibacterial potential of Saussurea obvallata petroleum ether extract
T2 - A spiritually revered medicinal plant
AU - Mishra, Abhay P.
AU - Saklani, Sarla
AU - Sharifi-Rad, Mehdi
AU - Iriti, Marcello
AU - Salehi, Bahare
AU - Maurya, Vineet Kumar
AU - Rauf, Abdur
AU - Milella, Luigi
AU - Rajabi, Sadegh
AU - Baghalpour, Navid
AU - Sharifi-Rad, Javad
N1 - Publisher Copyright:
© 2018 by the C.M.B. Association.
PY - 2018
Y1 - 2018
N2 - Uttarakhand Himalayan region holds Asteraceae or Compositae as the largest family of flowering, medicinal and aromatic plants. Species belonging to this family rises from low altitude to the alpine region. Among Asteraceae, Saussurea obvallata (DC.) Edgew. is widely used in several indigenous systems of medicine. Flowers, leaves and rhizomes of S. obvallata are used for several traditional, religious, therapeutic and ornamental purposes. Aim of this study was to determine the chemical composition and antibacterial efficacy of petroleum ether extract (PEE) of S. obvallata. Gas chromatography-mass spectrometry (GC-MS) analysis was used for identifying phytochemicals present in the plant extract. Furthermore, the PEE was assessed for in-vitro antibacterial activity against selected Gram positive and negative strains. Structure of squalene and α-linolenic acid methyl ester were identified in PEE by GC-MS analysis, by comparing the results obtained with NIST library and literature reports. PEE exhibited significant activity against Staphylococcus aureus, Escherichia coli, Bacillus cereus, Bacillus subtilis with IC50 value of 87.2 ± 1.6, 98.4 ± 1.1 and 90.2 ± 1.8 μg/ml, respectively. These results showed that squalene and a-linolenic acid derivative identified in S. obvallata may be responsible for the observed antibacterial activity. To the best of our knowledge, this is the first report focused on the chemical composition and antibacterial activity of S. obvallata.
AB - Uttarakhand Himalayan region holds Asteraceae or Compositae as the largest family of flowering, medicinal and aromatic plants. Species belonging to this family rises from low altitude to the alpine region. Among Asteraceae, Saussurea obvallata (DC.) Edgew. is widely used in several indigenous systems of medicine. Flowers, leaves and rhizomes of S. obvallata are used for several traditional, religious, therapeutic and ornamental purposes. Aim of this study was to determine the chemical composition and antibacterial efficacy of petroleum ether extract (PEE) of S. obvallata. Gas chromatography-mass spectrometry (GC-MS) analysis was used for identifying phytochemicals present in the plant extract. Furthermore, the PEE was assessed for in-vitro antibacterial activity against selected Gram positive and negative strains. Structure of squalene and α-linolenic acid methyl ester were identified in PEE by GC-MS analysis, by comparing the results obtained with NIST library and literature reports. PEE exhibited significant activity against Staphylococcus aureus, Escherichia coli, Bacillus cereus, Bacillus subtilis with IC50 value of 87.2 ± 1.6, 98.4 ± 1.1 and 90.2 ± 1.8 μg/ml, respectively. These results showed that squalene and a-linolenic acid derivative identified in S. obvallata may be responsible for the observed antibacterial activity. To the best of our knowledge, this is the first report focused on the chemical composition and antibacterial activity of S. obvallata.
KW - Ethnoreligious plants
KW - GC-MS
KW - MIC
KW - Squalene
KW - α-Linolenic acid
UR - https://www.scopus.com/pages/publications/85049965721
U2 - 10.14715/cmb/2018.64.8.10
DO - 10.14715/cmb/2018.64.8.10
M3 - Artículo
C2 - 29981685
AN - SCOPUS:85049965721
SN - 0145-5680
VL - 64
SP - 65
EP - 70
JO - Cellular and Molecular Biology
JF - Cellular and Molecular Biology
IS - 8
ER -